Protective effects of quercetin/moss extract (homalothecium sericeum hedw.) combination on STZ-induced diabetic rats

Abstract

Diabetes Mellitus (DM) is the most common chronic endocrine disorder. Although new drugs and administration techniques for diabetes were developed, the nature of complications that occur chronically and reduce patients’ quality of life have not been entirely determined. In our study, 35 male rats were used. A single dose of 40 mg/kg streptozotocin was injected intraperitoneal into 28 rats, and a total of five groups were formed. The diabetes model was performed by applying 40mg/kg STZ and was not established in the subjects in the control and citrate buffer groups with STZ solvent. The quercetin and moss extract were given by gavage for three weeks. The liver and pancreas tissues were examined by histochemical and immunohistochemical methods. Ki-67 (proliferation marker), antinuclear factor kappa (NF-κB), and interleukin 6 (IL-6) immunohistochemically staining revealed significant differences in inflammation and regeneration in the liver and pancreas tissues. In addition, the TUNEL staining method was used to determine apoptosis. Positivity was significant between the treatment and diabetes groups. It was determined that the agents used as treatment changed serum superoxide dismutase (SOD), malondialdehyde (MDA), C-peptide, insulin, and glucose levels.