Characterization and antibacterial activities of nickel oxide nanoparticles biosynthesis using walnut (carya Illinoensis) leaf extracts

Abstract

In this study, the biosynthesis of nickel oxide (NiO) nanoparticles and their antibacterial activities were investigated using walnut plant leaf extract. The synthesized nickel oxide (NiO) nanoparticles were analyzed using UV-Visible (UV-Vis), X-ray diffractometer (XRD) and transmission electron microscopy (TEM). The XRD graph provides information about the crystal structures of the (111), (200), (220), (311) and (222) planes. UV-Vis absorbance spectra give a single peak with a peak value of 345 nm. The peak value of the absorbance spectrum at 345 nm is related to Ni/NiO nanoparticles, and the fact that they give a single peak value is related to the spherical shape of the particle. As confirmed by TEM images, NiO nanoparticles have a spherical shape and an average size of 45.25 ± 9.50. The antibacterial activities of NiO nanoparticles were determined by the disk diffusion method, and NiO nanoparticles show more antibacterial properties against the gram-positive (+) bacteria Staphylococcus aureus than the gram-negative (-) bacteria Escherichia coli.