http://hdl.handle.net/20.500.12566/562 2026-04-05T22:45:16Z http://hdl.handle.net/20.500.12566/2393 The relationship between diabetes and myricetin: Bibliometric study Özcan, Filiz; Hangül, Ceren; Özdoğan, Hasan Myricetin is an active flavonoid compound known for its beneficial effects in diabetes management. The objective of this study is to characterize the research landscape on myricetin and diabetes by identifying its principal features, emerging trends, and thematic focuses thereby clarifying the therapeutic role of myricetin within the broader diabetes research field. Through bibliometric analysis, this study aims to provide comprehensive data specified to diabetes and myricetin. Material and Methods: R package “Bibliometrix” software was used to perform bibliometric analysis. The analysis included descriptive statistics, scientific mapping, keyword co-occurrence, and network analysis to identify influential elements such as highly cited articles, leading authors and journals, productive countries, and collaborative patterns. Results: The analysis showed limited growth until 2009, followed by rapid expansion. Most documents were original research articles (82.8%), highlighting active data generation. Keyword co-occurrence revealed oxidative stress, flavonoids, polyphenols, antioxidant, and insulin resistance as dominant themes. Twelve clusters were identified, with oxidative stress/myricetin/antioxidant and flavonoids/insulin resistance/phenolic compounds most prominent. Knekt et al. was most globally cited, Ong et al. most locally cited. China, India, Brazil, the United States, and Korea led output, while Journal of Ethnopharmacology, Molecules, and Plants Basel were most productive. Conclusion: The findings reveal a expanding body of research on myricetin in the context of diabetes, with increasing global interest, strong collaborative networks, and a clear thematic emphasis on oxidative stress and flavonoid-related mechanisms. This bibliometric overview provides a structured map of the field and may guide future studies towards unexplored research gaps.; Myricetin, diyabetin yönetiminde faydalı etkileriyle bilinen doğal bir flavonoid bileşiğidir. Bu çalışmanın amacı, myricetin ve diyabet konusundaki araştırma alanını karakterize ederek temel özelliklerini, ortaya çıkan eğilimleri ve tematik odak noktalarını belirlemek, böylece myricetinin terapötik rolünü diyabet araştırmaları bağlamında ortaya koymaktır. Bibliyometrik analiz yoluyla bu alana özgü kapsamlı veriler sunulması hedeflenmiştir. Gereç ve Yöntemler: Bibliyometrik analiz, R tabanlı ''Bibliometrix'' yazılım paketi kullanılarak gerçekleştirilmiştir. Analiz kapsamında betimleyici istatistikler, bilimsel haritalama, anahtar kelime birliktelik analizi ve ağ analizleri yapılmış; en çok atıf alan makaleler, önde gelen yazarlar ve dergiler, üretken ülkeler ve iş birliği örüntüleri belirlenmiştir. Bulgular: Analiz sonuçları, 2009 yılına kadar sınırlı bir yayın artışı olduğunu, bu tarihten sonra ise hızlı bir yükselişin başladığını göstermiştir. Yayınların çoğunluğunu özgün araştırma makaleleri (%82.8) oluşturmakta olup, bu durum alanda aktif veri üretimini yansıtmaktadır. Anahtar kelime analizinde oksidatif stres, flavonoidler, polifenoller, antioksidan ve insülin direnci öne çıkan temalar olmuştur. Toplam 12 tematik küme belirlenmiş; özellikle oksidatif stres/myricetin/antioksidan ve flavonoidler/insülin direnci/fenolik bileşikler kümeleri en belirgin gruplar olarak saptanmıştır. En fazla atıf alan çalışma Knekt ve ark. (2002), en yüksek yerel atıf alan çalışma ise Ong ve ark. (1997) olmuştur. Ülke bazında Çin, Hindistan, Brezilya, ABD ve Kore öne çıkmış; en üretken dergiler Journal of Ethnopharmacology, Molecules ve Plants-Basel olarak belirlenmiştir. Sonuç: Elde edilen bulgular, artan küresel ilgi, güçlü iş birliği ağları ve oksidatif stres ile flavonoid mekanizmalarına odaklı temaların myricetin ve diyabet konusundaki araştırmaların hızla genişleyen bir alan olduğunu göstermektedir. Bu bibliyometrik inceleme, alana ilişkin yapısal bir bakış sunmakta ve gelecekteki araştırmalar için olası boşlukların belirlenmesine katkı sağlamaktadır.. 2025-01-01T00:00:00Z http://hdl.handle.net/20.500.12566/2377 P1.03 Progesterone may be a regulator and B12 could be an indicator of the proximal D4Z4 repeat methylation status on 4q35ter Hangül, Ceren; Özcan, Filiz; Darbaş, Şule; Uysal, Hilmi; Koç, Ayşe Filiz; Berker Karaüzüm, Sibel FSHD patients have hypomethylation; different course in males and females was linked to sex hormones. We hypothesized that sex hormones, estradiol, testosterone, progesterone, and prolactin might be associated with methylation status of proximal part of D4Z4. We also investigated fT3, folic acid and vitB12 levels. DNA was extracted from 28 FSHD patients and 28 controls for bisulfite methylation analysis, and serum was separated for biochemical analysis of estradiol, testosterone, progesterone, prolactin, fT3, folic acid, and B12. Methylation analysis was specified to DR1, 5P regions, and proximal region covering both DR1 and 5P. Methylation was compared between patients and controls. Then correlation of methylation with estradiol, testosterone, progesterone, prolactin, fT3, folic acid, and B12 was investigated. We found that 5P and proximal region, but not DR1, were significantly hypomethylated in patients compared to controls. Male patients had significant hypomethylation compared to male controls. Older FSHD patients exhibited notable decrease in fT3 levels and hypomethylation of 5P region. Analyses of each CpG revealed seven hypomethylated positions significantly different from controls. Two of the positions demonstrated correlation with progesterone in controls. Except for one position, methylation levels were inversely correlated with B12 in patients. The results indicate that methylation of proximal D4Z4 region, particularly specific positions, may be associated with progesterone. https://www.fshdsociety.org/wp-content/uploads/2025/05/2025-IRC-ABSTRACT-BOOK.pdf 2025-01-01T00:00:00Z http://hdl.handle.net/20.500.12566/2376 Gut microbiota and short-chain fatty acid profiles in facioscapulohumeral dystrophy: Associations with epigenetic alterations Hangül, Ceren; Özyurt Koyuncu, Özlem; Baldi, Simone; Çolak, Dilek; Tokta, Öznur; Pekcan, Toygar; Bertorello, Sara; Pallecchi, Marco; Koç, Ayşe Filiz; Dalokay, Cumali; Uysal, Hilmi; Gülkesen, Kemel Hakan; Özcan, Filiz; Bartolucci, Gianluca; Berker Karaüzüm, Sibel; Amedei, Amedeo Gut microbiota (GM) affects muscle homeostasis, and growing evidence indicates dysbiosis of GM may be a contributing factor in the pathogenesis of dystrophies. Furthermore, GM metabolites can interact with DNA methylation. Facioscapulohumeral muscular dystrophy (FSHD) is the second common dystrophy with hypomethylation of DR1 and 5P regions of D4Z4 repeat on 4qter. Objective: Considering alteration of GM may be a contributing factor, we investigated (i) GM alterations and (ii) the correlation of microbial-derived free fatty acids (FFAs) with methylation of DR1 and 5P regions in FSHD. Methods: Twenty-eight FSHD patients and 28 gender-age-matched controls were included. GM characterisation was performed through 16S-rRNA sequencing. Methylation levels of DR1 and 5P regions were assessed by bisulphite sequencing. Faecal and circulating FFAs including short-chain fatty acids (SCFAs), medium-chain fatty acids (MCFAs) and long-chain fatty acids (LCFAs) were analysed with gas chromatography-mass spectrometry. Results: Altered GM was observed in patients, along with distinct profiles of faecal and circulating SCFAs, MCFAs and LCFAs. DR1 and 5P regions exhibited significant hypomethylation in FSHD compared to control. Hypomethylation correlated with faecal and circulating FFAs in patients, while no correlation was identified in healthy controls. The severely affected patients exhibited a notable increase in the prevalence of Pasteurellaceae, while the FFA profile was similar among mild and severely affected patients. This is the first study revealing that FSHD patients showed compositional and functional GM dysbiosis. A strong association between proximal D4Z4 hypomethylation with microbial-derived SCFAs was identified. Conclusion: These findings suggest that GM modulation with its metabolites could be a promising strategy for interventions in FSHD management. 2025-01-01T00:00:00Z http://hdl.handle.net/20.500.12566/2375 Progesterone may be a regulator and B12 could be an indicator of the proximal D4Z4 repeat methylation status on 4q35ter Hangül, Ceren; Özcan, Filiz; Darbaş, Şule; Uysal, Hilmi; Koç, Ayşe Filiz; Berker Karaüzüm, Sibel Facioscapulohumeral dystrophy (FSHD) has a hypomethylation-related epigenetic background and exhibits a different course in male and female patients. The differences between males and females have been linked to the levels of sex hormones. This study is the first to investigate the possible effect of these hormones on methylation status. We hypothesized that the levels of sex-related hormones, estradiol, testosterone, progesterone, and prolactin might be associated with the methylation status of the proximal part of the D4Z4. We also investigated the effect of fT3, folic acid, and vitamin B12 levels. We collected blood from 28 FSHD patients and 28 controls. DNA was extracted from each individual for bisulfite methylation analysis and serum was separated for biochemical analysis of estradiol, testosterone, progesterone, prolactin, fT3, folic acid, and B12 analysis. Methylation analysis was specified to the DR1, 5P regions and the proximal region covering both DR1 and 5P. Methylation levels were compared between FSHD patients and controls. The correlation of methylation levels with estradiol, testosterone, progesterone, prolactin, fT3, folic acid, and B12 was investigated. We found that the 5P region and the proximal region were significantly hypomethylated in FSHD patients compared to the controls, but not the DR1 region. Male patients exhibited a significant reduction in DNA methylation compared to male controls. Older FSHD patients exhibited a notable decrease in fT3 levels and hypomethylation of the 5P region. Analyses of each CpG revealed seven hypomethylated positions that were significantly different from the control group. Two of the positions demonstrated a correlation with progesterone in the control group. With the exception of one position, the methylation levels were inversely correlated with vitamin B12 in FSHD patients. The results of our study indicate that the methylation of the proximal D4Z4 region, particularly at specific positions, may be associated with progesterone. In addition, vitamin B12 may be an indicator of hypomethylation. We suggest that examining position-specific methylations may be a useful approach for the development of epigenetic treatment modalities. 2024-01-01T00:00:00Z