Tıbbi Laboratuvar Teknikleri Programı / Medical Laboratory Tecniques Program
http://hdl.handle.net/20.500.12566/558
2024-03-28T17:39:28ZEnvironmental toxicology and human health
http://hdl.handle.net/20.500.12566/1842
Environmental toxicology and human health
Demir, Eşref; Kacew, Sam
2024-01-01T00:00:00ZCrisis management in hospitals in the Covid-19 pandemic: the sample of Kahramanmaraş and Antalya
http://hdl.handle.net/20.500.12566/1791
Crisis management in hospitals in the Covid-19 pandemic: the sample of Kahramanmaraş and Antalya
Gökkaya, Elif Gözde
The World Health Organization was warned by the Chinese government for respiratory diseases of unknown cause on December 31, 2019. A week after the warning, it was reported that the cause of the epidemic was a new type of coronavirus and the disease was named Covid-19. On March 11, 2020, Covid-19 was declared a pandemic by WHO. The first Covid- 19 case in our country was seen on March 11, 2020 and accordingly, various arrangements were made to adapt to the developments in health institutions. The purpose of our research is to evaluate the views of health and administrative professionals working in hospitals in Turkey on Covid-19 process management and crisis management during the Covid-19 pandemic. The fact that there is no study in this direction in the literature reveals the original value of the study. In the research, health workers with and without administrative duties in private and public hospitals in Kahramanmaraş and Antalya were included. Different opinions were tried to be obtained by interviewing employees from different age groups and positions. In the study, 14 open-ended questions were asked to the participants and their answers were evaluated by content analysis. The interviewees were reached by snowball sampling method. The reason for this is that health personnel have problems in allocating time for the survey during their busy work schedule.
In the literature reviews, it has been noticed that the published studies on the crisis management followed by the hospitals in the world in the Covid-19 pandemic are limited. The results obtained within the framework of this limited literature were compared with the answers given by the survey participants. The low number of participants limits the study. The study can be detailed through interviews with different provinces and institutions. At the end of the study, it is found that there similarities between the Covid-19 crisis management in our country and the Covid-19 crisis management in the world. In addition, the participants stated that there were deficiencies in the crisis management and precautions taken in hospitals during the Covid-19 pandemic in our country, but all these problems were overcome with the least damage thanks to the superior dedication of the healthcare professionals.; 31 Aralık 2019 tarihinde Çin hükümeti tarafından nedeni bilinmeyen solunum hastalıkları için
Dünya Sağlık Örgütü’ne uyarıda bulunulmuştur. Uyarıdan bir hafta sonra salgın nedeninin
yeni tip koronavirüs olduğu rapor edilmiş ve hastalığa Covid-19 adı verilmiştir. 11 Mart 2020
tarihinde ise Covid-19 DSÖ tarafından pandemi ilan edilmiştir. Ülkemizde ilk Covid-19
vakası 11 Mart 2020 tarihinde görülmüş ve bu doğrultuda sağlık kuruluşlarında, yaşanan
gelişmelere uyum sağlamak için çeşitli düzenlemeler yapılmıştır. Araştırmamızın amacı,
Covid-19 pandemisinde Türkiye’deki hastanelerde çalışan sağlık ve idari personellerin Covid19 süreç yönetimi hakkındaki görüşlerini ve kriz yönetimini değerlendirmektir. Kriz yönetimi,
organizasyonun normal işleyişinin potansiyel riskini en aza indirmektir. Bu nedenle, kriz
yönetimi, krizin önlenmesi ve ortaya çıkması durumunda, ana paydaşların, kuruluş üyelerinin
ve yönetiminin eylem birliğini sağlayan organizasyonel değişiklikler yoluyla krizin
önlenmesini amaçlamaktadır.
Literatürde ülkemizdeki Covid-19 salgınına karşı hastanelerde geliştirilen kriz yönetimine
yönelik bir çalışmaya rastlanmamış olması çalışmanın özgün değerini ortaya çıkarmaktadır.
Araştırmada Kahramanmaraş ve Antalya’da bulunan özel ve kamu hastanelerinde idari görevi
olan ve olmayan sağlık çalışanlarına yer verilmiştir. Farklı yaş grubu ve pozisyondan
çalışanlarla görüşülerek farklı görüşler alınmaya çalışılmıştır. Araştırmada katılımcılara 14
açık uçlu soru yöneltilmiş ve yanıtları içerik analizi ile değerlendirilmiştir. Görüşme yapılan
kişilere kartopu örnekleme yöntemi ile ulaşılmıştır. Bunun sebebi, sağlık personelinin yoğun
iş temposu sırasında ankete zaman ayırmasında sorun yaşanmasıdır.
Bu çalışmada, dünyada Covid-19 pandemisiyle baş etmede hastanelerin aldığı önlemler ve
izledikleri kriz yönetimleri incelenmiştir. Yapılan literatür taramalarında, Covid-19
pandemisinde dünyadaki hastanelerin izlediği kriz yönetimi ile ilgili yayınlanmış çalışmaların
kısıtlı olduğu farkedilmiştir. Bu kısıtlı literatür çerçevesinde elde edilen sonuçlarla, anket
katılımcılarının verdiği cevaplar karşılaştırılmıştır. Katılımcı sayısının düşük olması yapılan
bu çalışmayı kısıtlamaktadır. Farklı il ve kurumlarla yapılacak görüşmelerle çalışma
detaylandırılabilir. Çalışma sonunda, ülkemizdeki Covid-19 kriz yönetimi ile dünyadaki
Covid-19 kriz yönetimi arasında benzerlikler olduğu bulunmuştur. Bunun yanında,
katılımcıların çoğunluğu ülkemizdeki Covid-19 pandemisi boyunca izlenen kriz yönetiminde
ve hastanelerde alınan önlemlerde eksiklikler olduğunu fakat tüm bu problemlerin sağlık
çalışanlarının üstün özverisi sayesinde en az hasarla atlatıldığını ifade etmişlerdir.
2023-01-01T00:00:00ZIn vivo evaluation of the neurogenotoxic effects of exposure to validamycin A in neuroblasts of drosophila melanogaster larval brain
http://hdl.handle.net/20.500.12566/1782
In vivo evaluation of the neurogenotoxic effects of exposure to validamycin A in neuroblasts of drosophila melanogaster larval brain
Turna Demir, Fatma; Demir, Eşref
Agriculture commonly utilizes crop protection products to tackle infestations from fungi, parasites, insects, and weeds. Validamycin A, an inhibitor of trehalase, possesses antibiotic and antifungal attributes. Epidemiological evidence has led to concerns regarding a potential link between pesticide usage and neurodegenerative diseases. The fruit fly, Drosophila melanogaster, has been recognized as a reliable model for genetic research due to its significant genetic similarities with mammals. Here, we propose to use D. melanogaster as an effective in vivo model system to investigate the genotoxic risks associated with exposure to validamycin A. In this study, we performed a neurotoxic evaluation of validamycin A in D. melanogaster larvae. Several endpoints were evaluated, including toxicity, intracellular oxidative stress (reactive oxygen species), intestinal damage, larval behavior (crawling behavior, light/dark sensitivity assay, and temperature sensitivity assay), locomotor (climbing) behavior, and neurogenotoxic effects (impaired DNA via Comet assay, enhanced by Endo III and formamidopyrimidine DNA glycosylase [FPG]). The results showed that exposure to validamycin A, especially at higher doses (1 and 2.5 mM), induced DNA impairment in neuroblasts as observed by Comet assay. Both larvae and adults exhibited behavioral changes and produced reactive oxygen species. Most importantly, this research represents a pioneering effort to report neurogenotoxicity data specifically in Drosophila larval neuroblasts, thus underscoring the importance of this species as a testing model in exploring the biological impacts of validamycin A. The in vivo findings from the experiments are a valuable and novel addition to the existing validamycin A neurogenotoxicity database.
2023-01-01T00:00:00ZPhotodynamic augmentation of oncolytic virus therapy for central nervous system malignancies
http://hdl.handle.net/20.500.12566/1753
Photodynamic augmentation of oncolytic virus therapy for central nervous system malignancies
Shimizu, Kazuhide; Kahramanian, Andranik; Jabbar, Muzammil Arif Din Abdul; Turna Demir, Fatma; Gokyer, Dilan; Uthamacumaran, Abicumaran; Rajan, Anant; Saad, Mohammad Ahsan; Gorham, Joshua; Wakimoto, Hiroko; Martuza, Robert L.; Rabkin, Samuel D.; Hasan, Tayyaba; Wakimoto, Hiroaki
Oncolytic viruses (OVs) have emerged as a clinical therapeutic modality potentially effective for cancers that evade conventional therapies, including central nervous system malignancies. Rationally designed combinatorial strategies can augment the efficacy of OVs by boosting tumor-selective cytotoxicity and modulating the tumor microenvironment (TME). Photodynamic therapy (PDT) of cancer not only mediates direct neoplastic cell death but also primes the TME to sensitize the tumor to secondary therapies, allowing for the combination of two potentially synergistic therapies with broader targets. Here, we created G47Δ-KR, clinical oncolytic herpes simplex virus G47Δ that expresses photosensitizer protein KillerRed (KR). Optical properties and cytotoxic effects of G47Δ-KR infection followed by amber LED illumination (peak wavelength: 585–595 nm) were examined in human glioblastoma (GBM) and malignant meningioma (MM) models in vitro. G47Δ-KR infection of tumor cells mediated KR expression that was activated by LED and produced reactive oxygen species, leading to cell death that was more robust than G47Δ-KR without light. In vivo, we tested photodynamic-oncolytic virus (PD-OV) therapy employing intratumoral injection of G47Δ-KR followed by laser light tumor irradiation (wavelength: 585 nm) in GBM and MM xenografts. PD-OV therapy was feasible in these models and resulted in potent anti-tumor effects that were superior to G47Δ-KR alone (without laser light) or laser light alone. RNA sequencing analysis of post-treatment tumor samples revealed PD-OV therapy-induced increases in TME infiltration of variable immune cell types. This study thus demonstrated the proof-of-concept that G47Δ-KR enables PD-OV therapy for neuro-oncological malignancies and warrants further research to advance potential clinical translation.
2023-01-01T00:00:00Z